Now in its third edition, Clinical Infectious Disease provides rapid access to an authoritative overview of practical clinical infectious disease topics including new chapters on medical microbiology, antimicrobial stewardship, and evolving treatments for COVID-19.

Chapters guide readers through suicide and oncolytic gene therapy, gene replacement and gene suppression therapy, vector development and refinement, immunogene therapy, TCR and CAR engineering, tumor vaccination using DNA or RNA vaccines, and antitumoral immune stimulation at different levels.

This Element discusses personalized drug screening as a novel patient stratification strategy for the determination of individualized treatment choices in oncology.

Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Chapters cover animal models of asthma, methods to measure asthma-related molecules, protocols to detect, isolate, culture and stimulate cells that contribute to asthma in mice and humans, methods to deplete these cells in vivo and study responses of intact airway tissues ex vivo.