The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function.

Antifreeze proteins enable organisms to avoid freezing under extreme conditions. The greatest diversity of known antifreeze proteins is in teleost fish. This volume contains articles from a wide spectrum of researchers on different areas of fish antifreeze research.

The intent of this book is to present a methodology for the formulation and computer implementation of mathematical models for multiple myeloma, a form of bone cancer. The models are stated as systems of ordinary and partial differential equations (ODE/PDEs).

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle.